High Quality Primer Purification - Purification Equipment for Oligo purification – Honya
High Quality Primer Purification - Purification Equipment for Oligo purification – Honya Detail:
Specification
| Compatible boards | 1, 3, 5, 8. |
| Filtration | blow filtration, suction filtration |
| Number of injection ports | 5, 6, 7, 8, 9, 10. |
| Compatible plate types | C18 plate, deep well plate, synthetic plate (compatible with most synthetic plates), microtiter plate |
| Module | single axis or dual axis |
| Voltage | 220V |
| Warranty | 1 year |
| Custom | Accepted |
Different ways of Purification
1. Gel Electrophoresis Chromatography Purification
Use denaturing polyacrylamide gel electrophoresis chromatography for purification. The denaturing agent is generally 4M formamide or 7M urea, the concentration of acrylamide is between 5-15%, and the proportion of methacrylamide is mainly between 2-10%.
After electrophoresis, the position of the nucleic acid band needs to be determined under the irradiation of ultraviolet light, the gel containing the target nucleic acid is cut off, the nucleic acid is smashed and leached, and then the leaching solution is concentrated, desalted, quantified and lyophilized.
2. DMT-On, HPLC Purification
Choose the DMT-On mode during the synthesis, the crude product was centrifuged and concentrated at room temperature to remove excess ammonia after the aminolysis.
Separation was performed using a C18 column with acetonitrile and 10% triethylamine-acetic acid (TEAA) as the eluent. After the elution is completed, it is concentrated, and then the DMT group is removed with trifluoroacetic acid. After neutralization, some salts and small molecules are removed through a cut-off tube, and finally desalted.
This method can obtain a product with higher purity, but it is necessary to pay attention to the occurrence of depurination.
3. DMT-Off, HPLC Purification
Choose the DMT-Off during the synthesis, and the crude product was centrifuged and concentrated at room temperature to remove excess ammonia after ammonolysis.
Separation was carried out using a C18 column with acetonitrile and 10% triethylamine-acetic acid in water as eluent. After the separation is completed and quantified, the aliquots are lyophilized.
This method requires careful adjustment of separation conditions, and can also obtain relatively pure target molecules.
Product detail pictures:
Related Product Guide:
"Sincerity, Innovation, Rigorousness, and Efficiency" is the persistent conception of our firm to the long-term to develop together with consumers for mutual reciprocity and mutual advantage for High Quality Primer Purification - Purification Equipment for Oligo purification – Honya , The product will supply to all over the world, such as: Panama, The Swiss, Sao Paulo, We are proud to supply our products to every costumer all around the world with our flexible, fast efficient services and strictest quality control standard which has always approved and praised by customers.
By Phyllis from Hungary - 2018.10.31 10:02
The customer service staff is very patient and has a positive and progressive attitude to our interest, so that we can have a comprehensive understanding of the product and finally we reached an agreement, thanks!
By Jill from Ecuador - 2017.04.08 14:55
